《植物生理学报》 2010, 46(2): 120-124

外源一氧化碳对铁胁迫下的离体水稻叶片氧化伤害的缓解作用

吴亭亭, 徐晟, 孙永刚, 吴洪洪, 沈文飚*

南京农业大学生命科学学院, 生命科学实验中心, 南京农业大学- 贝克曼离心机合作示范实验室, 南京 210095

通信作者：沈文飚；E-mail: wbshenh@njau.edu.cn；Tel: 025-84395 773

摘　要：

不同饱和度的外源 CO 水溶液预处理离体水稻叶片, 可以浓度依赖性地缓解铁胁迫引起的硫代巴比妥酸反应物质 (TBARS)含量的上升, 并诱导铁蛋白基因表达。此外, 50% 饱和度的 CO 水溶液还能不同程度地逆转由于柠檬酸铁胁迫引 起的叶中抗坏血酸过氧化物酶(APX)和超氧化物歧化酶(SOD)活性的上升。

关键词：离体水稻叶片; 铁胁迫; 一氧化碳; 氧化伤害; 铁蛋白

收稿：2009-10-15   修定：2009-11-30

Alleviation of Exogenous Carbon Monoxide on Iron-Induced Oxidative Damage in Detached Leaves of Oryza sativa L.

WU Ting-Ting, XU Sheng, SUN Yong-Gang, WU Hong-Hong, SHEN Wen-Biao*

College of Life Sciences, Laboratory Center of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique,Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing 210095, China

Corresponding author: SHEN Wen-Biao; E-mail: wbshenh@njau.edu.cn; Tel: 025-84395 773

Abstract:

In this report, pretreatment with different exogenous carbon monoxide (CO)-saturated aqueous solutions was not only able to dose-dependently alleviate the increase of thiobarbituric acid reactive substances (TBARS) content in Fe-citrate-stressed detached rice leaves, but also to induce the expression of ferritin genes. Further research also showed that 50% CO-saturated aqueous solution could block differentially the enhancement of ascorbate peroxidase (APX) and superoxide dismutase (SOD) activities.

Key words: detached rice leaves; iron stress; carbon monoxide; oxidative damage; ferritin